Authors
Harsha Sharma
Abstract
Proteomics is defined as the study of interaction of cell and proteins. Proteomics is a useful tool in shifting from genomics to the complimentary protein of human within the wide field of functional OMICS. Proteomics is expected to produce better abberation markers for diagnosis and therapy of various deadly diseases because the proteome reflects more precisely on the basis of dynamic state of cell, tissue, or organism. The present review study is on the technologies of proteomics and their technologies and their applications for crop improvement.Mass spectroscopy has become an significant tool in the field of molecular and cellular biology and plays an important role in proteomics.The advent of proteomics has opened new doors for opportunities and challenges for those who are keen to know the depth of this field. Keywords: Bioinformatics, Mass Spectroscopy, Proteomics.
Introduction
In the area of therapeutics, the rapid momentum gaind in certain years is with the help of proteomics developed recently. Proteomics study protiens especially the composition, structures, functions and their interactions which directs the activity of the cell. Due to the frequent change in the protein expression profile, micro and macro environmental conditions, Proteomics is more complex than genomics (Holman et al., 2013). It is expected to provide direct understanding of functions and regulation. It is necessary to identify the genes and protiens that controls crop architecture or stress resistance to facilitate the biologically driven improvement in crop productivity. Therefore the main aim of this review is proteomics technologies and its application for crop improvements.
Types of Proteomics
Proteomics are divided into different categories which are based upon the responses of proteins under stress conditions
1. Expression Proteomics
The qualitative and quantitative expression of total proteins are studied by the expression proteomics under two different conditions. The expressional changes which are present and absent in tumour tissue as compare to normal tissue is observed by the 1-D gel electrophoresis, mass spectrometry techniques.
2. Structural Proteomics
Stuctural proteomics studies three dimensional shape and structure of complex functional protiens.This helps in determining the same in differet cell organelles. X-Ray crystallography and NMR spectroscopy can be used in this.
References
Ahmad, Parvaiz, et al. “Role of Proteomics in Crop Stress Tolerance.” Frontiers in Plant Science, vol. 7, Aug. 2016.
Aslam, Bilal, et al. “Proteomics: Technologies and Their Applications.” Journal of Chromatographic Science, vol. 55, no. 2, Oct. 2016, pp. 182–196.
Beyene, Belachew, et al. “Review on Proteomics Technologies and Its Application for Crop Improvement.” Innovative Systems Design and Engineering, vol. 7, no. 6, 2016, pp. 7–15.
Chandrasekhar, K., et al. “A Short Review on Proteomics and Its Applications.” International Letters of Natural Sciences, vol. 17, 2014, pp. 77–84.
Holman, J. D., Dasari, S. and Tabb, D. L., 2013. Informatics of protein and posttranslational modification detection via shotgun proteomics. Methods Mol. Biol.1002, 167–179.
Jacoby, R. P., Millar, A. H. and Taylor, N. L., 2013b. Application of selected reaction monitoring mass
spectrometry to field-grown crop plants to allow dissection of the molecular mechanisms of a biotic stress
tolerance. Front. Plant Sci.4:20.
Komatsu, Setsuko, and Zahed Hossain. “Preface—Plant Proteomic Research.” International Journal of
Molecular Sciences, vol. 18, no. 1, Apr. 2017, p. 88.
Lilley, K. S, and Paul Dupree. “Methods of Quantitative Proteomics and Their Application to Plant
Organelle Characterization.” Journal of Experimental Botany, vol. 57, no. 7, 2006, pp. 1493–1499.
How to cite this article?
| APA Style | Sharma, H. (2019). Proteomics Technologies and its Application for Crop Improvement. Academic Journal of Botanical Sciences, 1(1), 16-19. |
| Chicago Style | |
| MLA Style | |
| DOI | |
| URL |