Authors
Ruchika Dwivedi, Dr. Munish Mishra
Abstract
Various pesticides and insecticides have been used in every region of most of the countries in the world to manage and mitigate different agricultural pests. Predicament of the presence of pesticide residues in fruits and vegetables is the major concern to the staid matter of individual health and wellbeing. The present study entitled “Extraction and Identification of Pesticide residue in Fruits and Vegetable samples for Forensic consideration” was carried out with an objective to analyze and identify pesticide residue in fruits and vegetables by using QuEChERS extraction procedure and TLC method. 4 Fruits (banana, apple, papaya, and guava) and 6 vegetable (eggplant, carrot, bell pepper, potato, okra, cauliflower) sample were collected from vegetable market of Allahabad. Extraction of pesticides was done by using QuEChERS extraction procedure and analyzed for qualitative determination of the presence of pesticides on samples by using thin-layer chromatography. Result revealed that 90% of samples were positively contaminated with as a minimum of one or more pesticide. The findings of the study might help in extending awareness to the farmers and local people about pesticides and their hazardous effects on humans. Whereas the methodology might be useful to the analyst working in the particular field as well as a forensic laboratory for rapid extraction of residues from biological and non-biological samples and to overcome the major issue of all the forensic laboratories i.e. pendency of cases. Keywords: TLC, QuEChERS, Chlorpyrifos, Carbofuran, Cypermethrin.
Introduction
The Environmental Protection Agency (EPA) defines the term pesticide as the use of any substance or combination of substances proposed for preventing, repelling, destroying, or extenuating any pest. The definition of pesticide according to the Food and Agriculture Organization (FAO) is very specific which ascertain that any substance or combination of substances which are purposefully used for controlling or preventing or even destroying any kind of pest including organism or pest which are capable of carrying any animal, plant or human disease; any plant or animal species which are capable of causing any kind of harm during manufacturing, processing, storage, transportation or even selling of any kind of food or farming products, any kind of coppice or even animal feedstuff; any substance or blend of substances which may be given to any animal for purpose of controlling or destroying any kind of pest, arachnids or insects living inside or even outside of the body of that animal (Zacharia and Tano, 1-18). India ranks 12th in Asia and is being the largest producer in the world for using pesticides. Most of the population of India is majorly dependent on agriculture which requires the need for using pesticides to prevent the loss of agriculture products from any kind of pests. When we see the comparative use of pesticides in a global scenario, India is the country whose average consumption of pesticides in agricultural use is comparatively lower than any other developed country. Even then, the presence of pesticide residue in post harvested agricultural products is at great height (Abhilash and Nandita, 1-12). The use of pesticides and other fertilizer has helped to a great extent to the farmers with an increase in yielding crop productivity but consequently, their bad effect on nature and human health is also unavoidable. Pesticides have been classified into 4 major categories with include Organophosphate Pesticide: These pesticides are basically affecting the activity of the enzyme which regulates the secretion of acetylcholine which is a neurotransmitter and in turn affects the nervous system. These pesticides show similar effects in both, insects and as well as humans. (Cabello et al., 471-479). Carbamate Pesticides: The effects of carbamate pesticides are quite similar to the effects of organophosphate pesticides as it also affects the activity of neurotransmitter enzyme acetylcholinesterase which in turn affects the nervous system. The effects of the enzyme are generally reversible (Fernandez et al., 43-56). Organochlorine Insecticides: these insecticides were generally being used in the ancient times, but a lot of them have been aloof from the commercial market because of their persistency in nature and hazards related to the human health as well as the environment the most common example of this group of pesticides are DDT and chlordane and many more (Pandit et al., 240-243). Pyrethroid Pesticides: These are generally synthesis from a natural pesticide “pyrethrin” found in the algae known as chrysanthemum. They have been adapted to augment their stability in the atmosphere. Some of these synthetically prepared pyrethroids are noxious to the nervous system (Duara et al., 43-46).
References
Abhilash, P. C., and Nandita Singh. “Pesticide Use and Application: An Indian Scenario.” Journal of Hazardous Materials 2009: 1–12.
Anastassiades, Michelangelo, et al. “Fast and Easy Multiresidue Method Employing Acetonitrile Extraction/Partitioning and ‘Dispersive Solid-Phase Extraction’ for the Determination of Pesticide Residues in Produce.” Journal of AOAC INTERNATIONAL, vol. 86, no. 2, 2003, pp. 412–431.
Cabello, Gertrudis et al. “A Rat Mammary Tumor Model Induced by the Organophosphorous Pesticides Parathion and Malathion, Possibly through Acetylcholinesterase Inhibition.” Environmental Health Perspectives 109.5 (2001): 471–479.
Coulson, D. M. “A General Approach to Pesticide Residue Analysis.” Journal of Forensic Sciences, vol. 17, no. 4, 1972, pp. 47-56.
Dasika, Rohan. “Pesticide Residue Analysis of Fruits and Vegetables.” Journal of Environmental Chemistry and Ecotoxicology, vol. 4, no. 2, 2012, pp. 19–28.
Duara, B et al. “Residues of Cypermethrin and Fenvalerate on Brinjal.” Pesticide Research Journal 15.1 (2003): 43–46.
Khan, Muhammad Saqib et al. “Assessment of Pesticide Residues on Selected Vegetables of Pakistan.” Journal of the Chemical Society of Pakistan 33.6 (2011): 816–821.
Kumari, D., and S. John. “Health Risk Assessment of Pesticide Residues in Fruits and Vegetables from Farms and Markets of Western Indian Himalayan Region.” Chemosphere, vol. 224, 2019, pp. 162–167.
Latif, Yawar, et al. “Monitoring of Pesticide Residues in Commonly Used Fruits in Hyderabad Region, Pakistan.” American Journal of Analytical Chemistry, vol. 02, no. 08, 2011, pp. 46–52.
Mahmood, Adeel et al. “Human Health Risk Assessment and Dietary Intake of Organochlorine Pesticides through Air, Soil and Food Crops (Wheat and Rice) along Two Tributaries of River Chenab, Pakistan.” Food and Chemical Toxicology 71 (2014): 17–25.
Montgomery, M. P., et al. “Incident Diabetes and Pesticide Exposure among Licensed Pesticide Applicators: Agricultural Health Study, 1993-2003.” American Journal of Epidemiology, vol. 167, no. 10, 2008, pp. 1235–1246.
Pandit, G. G. et al. “Distribution and Fate of Persistent Organochlorine Pesticides in Coastal Marine Environment of Mumbai.” Environment International. Vol. 32. N.p., 2006. 240–243.
Shafi, Muhammad et al. “A Study of Pesticide Residues in Different Fruits Collected from Different Fruit Markets of Lahore, Punjab.” Journal of Agro alimentary Processes and Technologies 20.4 (2014): 298–303.
Zacharia and James Tano. “Identity, Physical and Chemical Properties of Pesticides.” Pesticides in the Modern World - Trends in Pesticides Analysis, 2011, pp. 1–18.
How to cite this article?
APA Style | Dwivedi, R., & Mishra, Dr. M. (2020). Extraction and Identification of Pesticide Residue in Fruits and Vegetable Samples for Forensic Consideration. Academic Journal of Forensic Sciences, 03(02), 01-09. |
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