Authors
Anu Priya Singh, Priya Singh, Dr. Vaibhav Saran
Abstract
In crime scene, mostly blood, semen, saliva, urine, vaginal fluid, sweat etc. are mostly found in case of sexual assault, theft, burglary, murder, suicide, homicide, road accident etc. these evidence play major role in criminal investigation as they can help to link criminal with crime as act of Locard principal of exchange. This reviewed paper discuss about the different techniques for the identification of blood protein in the body fluids. Also explains the difference between the body fluids on the basis of specific protein present in all body fluids. Keywords: Blood Protein, Body Fluids, SDS-PAGE, MALDI-TOF.
Introduction
Blood
Blood is a body fluid in humans it is compose of blood cell and blood plasma. Blood cell constitutes 45% of blood fluid. Protein are also called plasma protein which constitutes 55% of blood fluid. It is essentially an aqueous solution containing 92% water 8% blood plasma proteins. Plasma contain verity of protein like albumin globulin fibrinogen. Blood protein serves different function including transport of lipid, hormone, and vitamins, mineral and functioning of immune system, it also acts as enzyme.
Blood Protein
Determination of the protein constituents of human plasma has been an active area of research for several years1Proteins are composed of individual units called amino acids2. Hundreds of proteins are dissolved in the plasma, Protein identification is an integral part of proteomics research.3.The analysis of proteins within forensic science is a seldom documented 4. In crime scene mostly blood semen saliva urine vaginal fluid, sweat etc. are mostly found in case of sexual assault theft burglary murder suicide homicide road accident etc. these evidence play major role in criminal investigation as they can help to link criminal with crime as act of locard principal of exchange5-7. The application of protein analyses that are more sophisticated and sensitive than those technique currently utilized within forensic science 8. Protein studied have maximum used in medical science by measuring the concentration of these proteins, the clinician can obtain information regarding disease states of human 9-16. Human Serum contains several proteins which have various concentration but bulk of protein is lower. molecular weight protein
References
Adkins JN, Varnum SM, Auberry KJ, Moore RJ, Angell NH, Smith RD, Springer DL, Pounds JG, 2002 Toward a Human Blood Serum Proteome Analysis By Multidimensional Separation Coupled With Mass Spectrometry Molecular & Cellular Proteomics 1 (12): 947–955
Aebersold R., Mann M., 2003 Mass spectrometry-based proteomics Nature vol 422, pages198–207
Ahmad Y, Sharma N (2009) An Effective Method for the Analysis of Human Plasma Proteome using Two-dimensional Gel Electrophoresis. J Proteomics Bioinform 2: 495-499. doi:10.4172/jpb.1000111
Allery JP, Telmon N, Blanc A, Mieusset R, Rouge D (2003) Rapid detection of sperm: comparison of two methods Journal of Clinical Forensic Medicine 10(1):5–7. doi:10.1016/S1353-1131(02)00158-X
Anderson L., Anderson NG.,1977 High resolution two-dimensional electrophoresis of human plasma proteins Proc. Natl. Acad. Sci. USA Vol. 74, No. 12, pp. 5421-5425
Aroca-Aguilar J-D, Ferna´ndez-Navarro A,Ontaño´n J, Coca-Prados M, Escribano J (2018) Identification of myocilin as a blood plasma protein and analysis of its role in leukocyte adhesion to endothelial cell monolayers PLoS ONE 13(12):e020936
Burnette, W. N. (1981) “Western Blotting”: Electrophoretic transfer of proteins from sodium dodecyl sulfate-polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A Analytical Biochemistry. 112:195-203
Cameron AT., JS., Guthrie., White FD., 1936 Estimation Of Proteins Of Blood Plasma The Canadian Medical Association Journal
Cottrell JS., 2011 Protein identification using MS/MS data Journal of proteomics 74(10):1842-51
Cristea IM., Gaskell SJ., and Whetton AD., 2004 Proteomics techniques and their application to hematology Blood Volume 103, Number 10
Danielson PB., 2011 Isolation of Highly Specific Protein Markers for the Identification of Biological Stains: Adapting Comparative Proteomics to Forensics NCJRS DN-BX-K001
Deininger L.,(2018) Multi-Informative and Specific Detection of Blood in
Fingermarks via MALDI-MS Based Strategies Sheffield Hallam University
Deininge L., Patel E.,Clench MR, Sears V., Sammon C., Francese S.,2 016 Proteomics goes forensic: detection and mapping of blood signatures in fingermarks Sheffield Hallam University Research Archive Proteomics 16 (11-12), 1707-1717
How to cite this article?
| APA Style | Singh, A. Singh, P. and Saran, V. (2019). Critical Review on Role of Blood Protein in Forensic Science. Academic Journal of Forensic Sciences, 2(2), 26-35. |
| Chicago Style | |
| MLA Style | |
| DOI | |
| URL |